Filter paper test with seed freezing
The seeds were placed in Petri dishes, on six layers of filter paper soaked with water. Depending on the combination, it was either tap water, Borucin or ID’EAU water. Subsequently, the seeds were placed for 3 days in a dark chamber at a temperature of 20oC. After incubation, they were moved into cold storage for 24 hours, for freezing at a temperature of -20oC.
The next stage was placing the dishes with the seeds in a room at a temperature of 20oC, under NUV lamps (40 W Philips) with 320 – 400 nm wavelength, where they were subjected to alternated lighting, 12 hours of light and 12 hours of darkness. During the test, 0.5 ml of the tested water was added to each dish after 3 and 6 days. Each combination was executed in 5 repeats (40 seeds), 200 seeds in total.