Biological Parameters

Filter paper test with seed freezing

The seeds were placed in Petri dishes, on six layers of filter paper soaked with water. Depending on the combination, it was either tap water, Borucin or ID’EAU water. Subsequently, the seeds were placed for 3 days in a dark chamber at a temperature of 20oC. After incubation, they were moved into cold storage for 24 hours, for freezing at a temperature of -20oC.

The next stage was placing the dishes with the seeds in a room at a temperature of 20oC, under NUV lamps (40 W Philips) with 320 – 400 nm wavelength, where they were subjected to alternated lighting, 12 hours of light and 12 hours of darkness. During the test, 0.5 ml of the tested water was added to each dish after 3 and 6 days. Each combination was executed in 5 repeats (40 seeds), 200 seeds in total.

Seed health assessment

Fungal colonisation of the studied seed batches was assessed after 10 days, using a ZEISS Stemi 2000 stereoscopic microscope, at a 50-60x magnification. The basis for fungus identification was the pattern of conidiophore branching, arrangement and appearance of spores, presence of acervuli and pycnidia.

Health assessment of Amsterdam variety carrot seeds


The tests were performed at the Poznań University of Life Sciences, Faculty of Horticulture and Landscape Architecture

Woda kranowa Tap water
Woda Borucin Borucin water
Woda ID’eau ID’eau water
Grzyb Epicoccum nigrum Fungus Epicoccum nigrum
Grzyb Stemphylium spp. Fungus Stemphylium spp.
Grzyb Ulocladium consortiale Fungus Ulocladium consortiale

After saturating the carrot seeds with ID’EAU water, in comparison with the control samples – Borucin water and tap water – it was determined that:

  • a markedly lower number of seeds were colonised by the fungus Epicoccum nigrum,
  • the fungus Stemphylium botryosum was absent on the surface of the seeds,
  • 6-fold decrease in the presence of the fungus Ulocladium consortiale, compared to Borucin water.